پیوندهای مفید
آمار
| تعداد نشریات | 9 |
| تعداد شمارهها | 447 |
| تعداد مقالات | 5,734 |
| تعداد مشاهده مقاله | 8,047,193 |
| تعداد دریافت فایل اصل مقاله | 6,547,393 |
بررسی اثر هندسهی داربست مهندسی بافت استخوان بر مدولاسیون مکانیکی رفتار لایه سلولی | ||
| نشریه مهندسی مکانیک امیرکبیر | ||
| مقاله 2، دوره 51، شماره 3، مرداد و شهریور 1398، صفحه 11-20 اصل مقاله (1.68 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22060/mej.2018.13215.5571 | ||
| نویسندگان | ||
| امیرعلا بخشیان نیک؛ بهمن وحیدی* | ||
| دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، ایران | ||
| چکیده | ||
| پیشرفت در روشهای تولید افزایشی تاثیر چشمگیری در امکان کنترل و اصلاح طرح داخلی داربست استخوانی و ویژگیهای آن گذاشته است. این امر موجب ارائهی روشهای نوین برای طراحی داربستهای مهندسی بافت استخوان شده است. طراحی کامپیوتری داربستهای مبتنی بر سطوح مینیمال مثلثاتی به دلیل نسبت سطح به حجم بالا که عاملی حیاتی در پژوهشهای زیستی است، مطرح میباشد. از آنجایی که تحریکهای مکانیکی اعمال شده در حین عبور سیال از داخل تخلخلهای داربست، بر تکثیر، مهاجرت، تمایز و سرنوشت سلولهای بنیادی مزانشیمی اثر دارد و این تحریکهای مکانیکی خود متاثر از هندسهی داخلی داربست هستند. در این پژوهش نگاهی دقیقتر به این موضوع افکنده میشود و با استفاده از ابزار دینامیک سیالات محاسباتی دو داربست مهندسی بافت استخوان مبتنی بر سطوح مینیمال مثلثاتی با نامهای G و I از منظر مدولاسیون مکانیکی و برهمکنش با لایهی سلولی به ضخامت 8/5 میکرومتر که نمایندهی تجمع سلولی روی داربست است، مورد بررسی قرار میگیرد. داربست G به دلیل هندسهی داخلی مناسب و ایجاد توزیع تنش برشی مناسب روی لایهی سلولی شرایط بهتری را برای کشت سلول و برهمکنش سیال-سازه ایجاد میکند. از سویی دیگر در داربست I نقاط مردهای ایجاد میشود که یکنواختی سیگنالدهی در سطح آن را محدود میکند. | ||
| کلیدواژهها | ||
| داربست مهندسی بافت استخوان؛ دینامیک سیالات محاسباتی؛ لایهی سلولی؛ مدولاسیون مکانیکی؛ سلول بنیادی مزانشیمی | ||
| عنوان مقاله [English] | ||
| The Effect of Bone Tissue Engineering Scaffold Architecture on Mechanical Modulation of Cell Layer Behavior | ||
| نویسندگان [English] | ||
| Amirala Bakhshian Nik؛ Bahman Vahidi | ||
| MSc/University of Tehran | ||
| چکیده [English] | ||
| Advances in Additive Manufacturing (AM) techniques have made the design, control and modification of bone scaffolds inner architectures and their mechanical properties possible. CAD bone scaffolds based on triply periodic minimal surfaces (TPMSs) have attracted attentions, due to their high surface area to volume ratio pore interconnectivity which enhance cell migration and attachment. The mechanical stimuli acting while fluid is flowing through scaffold pores can influence on proliferation, migration, differentiation and fate of mesenchymal stem cell. In the present study, the interaction between 2 TPMS-based bone scaffolds, termed G and I, with fluid in the presence 8.5 μm-cell layer (as mesenchymal stem cell accumulation) have been evaluated computationally. The results demonstrated that the scaffold G can modulate the cells more adequate due to producing homogenous distribution of mechanical stimuli comparing to scaffold I. The range of shear stress and von Mises stress for scaffold G are not wide which means the cells are sensing roughly the same mechanical stimuli. For both scaffolds in inlet velocities less than 50 μm/s, the magnitude of stresses is negligible. In addition, for scaffold I, there are dead zones which mechanical stimuli are approximately zero which prevents dynamic cell culture and homogenous signaling. | ||
| کلیدواژهها [English] | ||
| Bone Tissue Engineering Scaffold, Computational Fluid Dynamics, Cell Layer, Mechanical Modulation, Mesenchymal Stem Cell | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
[1] A. B. Nik, B. Vahidi, The effect of bone scaffold gradient architecture design on stem cell mechanical modulation: a computational study, in 2015 22nd Iranian Conference on Biomedical Engineering (ICBME), 2015, pp. 309-313. [2] C. Landsberg, F. Stenger, A. Deutsch, M. Gelinsky, A. Rösen-Wolff, A. Voigt, Chemotaxis of mesenchymal stem cells within 3D biomimetic scaffolds—a modeling approach, Journal of biomechanics, vol. 44, pp. 359-364, 2011. [3] C. Sandino, J. Planell, D. Lacroix, A finite element study of mechanical stimuli in scaffolds for bone tissue engineering, Journal of biomechanics, vol. 41, pp. 1005-1014, 2008. [4] S. Wu, X. Liu, K. W. Yeung, C. Liu, X. Yang, Biomimetic porous scaffolds for bone tissue engineering, Materials Science and Engineering: R: Reports, vol. 80, pp. 1-36, 2014. [5] C. R. Kothapallim, R. D. Kamm, 3D matrix microenvironment for targeted differentiation of embryonic stem cells into neural and glial lineages, Biomaterials, vol. 34, pp. 5995-6007, 2013. [6] R. Voronov, S. VanGordon, V. I. Sikavitsas, D. V. Papavassiliou, Computational modeling of flow-induced shear stresses within 3D salt-leached porous scaffolds imaged via micro-CT, Journal of biomechanics, vol. 43, pp. 1279-1286, 2010. [7] E. Baas, J. H. Kuiper, Y. Yang, M. A. Wood, A. J. El Haj, In vitro bone growth responds to local mechanical strain in three-dimensional polymer scaffolds, Journal of biomechanics, vol. 43, pp. 733-739, 2010. [8] M. J. Song, D. Dean, M. L. K. Tate, Mechanical modulation of nascent stem cell lineage commitment in tissue engineering scaffolds, Biomaterials, vol. 34, pp. 5766-5775, 2013. [9] D. Li, T. Tang, J. Lu, and K. Dai, "Effects of flow shear stress and mass transport on the construction of a large-scale tissue-engineered bone in a perfusion bioreactor," Tissue Engineering Part A, vol. 15, pp. 2773-2783, 2009. [10] F. Vermolen, A. Gefen, Semi-stochastic cell-level computational modelling of cellular forces: application to contractures in burns and cyclic loading, Biomechanics and modeling in mechanobiology, vol. 14, pp. 1181-1195, 2015. [11] J. Will, R. Detsch, A. Boccaccini, Chapter 7.1-Structural and Biological Characterization of Scaffolds, Characterization of Biomaterials, Academic Press, Oxford, pp. 299-310, 2013. [12] F. P. Melchels, A. M. Barradas, C. A. Van Blitterswijk, J. De Boer, J. Feijen, D. W. Grijpma, Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing, Acta Biomaterialia, vol. 6, pp. 4208-4217, 2010. [13] R. Gabbrielli, I. Turner, C. R. Bowen, Development of modelling methods for materials to be used as bone substitutes, in Key Engineering Materials, 2007, pp. 903-906. [14] H. Sun, F. Zhu, Q. Hu, P. H. Krebsbach, Controlling stem cell-mediated bone regeneration through tailored mechanical properties of collagen scaffolds, Biomaterials, vol. 35, pp. 1176-1184, 2014. [15] C. Bandeiras, A. Completo, A. Ramos, Influence of the scaffold geometry on the spatial and temporal evolution of the mechanical properties of tissue-engineered cartilage: insights from a mathematical model, Biomechanics and modeling in mechanobiology, vol. 14, pp. 1057-1070, 2015. [16] S. Giannitelli, D. Accoto, M. Trombetta, A. Rainer, Current trends in the design of scaffolds for computer-aided tissue engineering, Acta biomaterialia, vol. 10, pp. 580-594, 2014. [17] N. C. Pearson, S. L. Waters, J. M. Oliver, R. J. Shipley, Multiphase modelling of the effect of fluid shear stress on cell yield and distribution in a hollow fibre membrane bioreactor, Biomechanics and modeling in mechanobiology, vol. 14, pp. 387-402, 2015. [18] D.-J. Yoo, Computer-aided porous scaffold design for tissue engineering using triply periodic minimal surfaces, International Journal of Precision Engineering and Manufacturing, vol. 12, pp. 61-71, 2011. [19] D.-J. Yoo, Advanced porous scaffold design using multi-void triply periodic minimal surface models with high surface area to volume ratios, International Journal of Precision Engineering and Manufacturing, vol. 15, pp. 1657-1666, 2014. [20] L. Elomaa, S. Teixeira, R. Hakala, H. Korhonen, D. W. Grijpma, J. V. Seppälä, Preparation of poly (ε-caprolactone)-based tissue engineering scaffolds by stereolithography, Acta Biomaterialia, vol. 7, pp. 3850-3856, 2011. [21] M. A. Woodruff, D. W. Hutmacher, The return of a forgotten polymer—polycaprolactone in the 21st century, Progress in Polymer Science, vol. 35, pp. 1217-1256, 2010. [22] K. Van de Velde, P. Kiekens, Biopolymers: overview of several properties and consequences on their applications, Polymer testing, vol. 21, pp. 433-442, 2002. [23] H. Yu, C. Y. Tay, W. S. Leong, S. C. W. Tan, K. Liao, L. P. Tan, Mechanical behavior of human mesenchymal stem cells during adipogenic and osteogenic differentiation, Biochemical and biophysical research communications, vol. 393, pp. 150-155, 2010. [24] F. Zhao, T. J. Vaughan, L. M. Mcnamara, Multiscale fluid–structure interaction modelling to determine the mechanical stimulation of bone cells in a tissue engineered scaffold, Biomechanics and modeling in mechanobiology, vol. 14, pp. 231-243, 2015. [25] A. L. Olivares, È. Marsal, J. A. Planell, D. Lacroix, Finite element study of scaffold architecture design and culture conditions for tissue engineering, Biomaterials, vol. 30, pp. 6142-6149, 2009. [26] A. B. Yeatts and J. P. Fisher, "Bone tissue engineering bioreactors: dynamic culture and the influence of shear stress," Bone, vol. 48, pp. 171-181, 2011. [27] S. D. Subramony, A. Su, K. Yeager, and H. H. Lu, "Combined effects of chemical priming and mechanical stimulation on mesenchymal stem cell differentiation on nanofiber scaffolds," Journal of biomechanics, vol. 47, pp. 2189-2196, 2014. [28]T. Nagel, D. J. Kelly, Mechano-regulation of mesenchymal stem cell differentiation and collagen organisation during skeletal tissue repair, Biomechanics and modeling in mechanobiology, vol. 9, pp. 359-372, 2010. [29] F. Guilak, D. L. Butler, S. A. Goldstein, F. P. Baaijens, Biomechanics and mechanobiology in functional tissue engineering, Journal of biomechanics, vol. 47, pp. 1933-1940, 2014. [30] L. M. Kock, J. Malda, W. J. Dhert, K. Ito, D. Gawlitta, Flow-perfusion interferes with chondrogenic and hypertrophic matrix production by mesenchymal stem cells, Journal of biomechanics, vol. 47, pp. 2122-2129, 2014. [31] M. S. Hossain, D. Bergstrom, X. Chen, Computational modelling of the scaffold-free chondrocyte regeneration: a two-way coupling between the cell growth and local fluid flow and nutrient concentration, Biomechanics and modeling in mechanobiology, vol. 14, pp. 1217-1225, 2015. | ||
|
آمار تعداد مشاهده مقاله: 1,793 تعداد دریافت فایل اصل مقاله: 1,458 |
||
