پیوندهای مفید
آمار
| تعداد نشریات | 9 |
| تعداد شمارهها | 452 |
| تعداد مقالات | 5,748 |
| تعداد مشاهده مقاله | 8,237,231 |
| تعداد دریافت فایل اصل مقاله | 6,756,317 |
طراحی و مدلسازی یک ریزجداکننده دی الکتروفورزیس جدید دو طبقه مبتنی بر ریزسیالات برای جداسازی کارآمد سلولهای تومور گردشی | ||
| نشریه مهندسی مکانیک امیرکبیر | ||
| دوره 57، شماره 7، مهر 1404، صفحه 845-868 اصل مقاله (4.05 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22060/mej.2025.24650.7892 | ||
| نویسندگان | ||
| رضا حاجی آقائی وفائی* ؛ الناز پوررضا | ||
| دانشکده مهندسی برق، دانشگاه بناب، بناب ، ایران | ||
| چکیده | ||
| جداسازی قابل اطمینان سلولهای تومور گردشی از سلولهای خونی برای تحلیل و پیشبینی زودهنگام سرطان امری حیاتی محسوب میشود. هدف از این پژوهش، جداسازی سلولهای تومور گردشی و زیرگونههای سلولهای سفید خون (گرانولوسیتها و T-لنفوسیتها) با بهکارگیری نیروی دی الکتروفورزیس است. دی الکتروفورزیس به حرکت یک ذره خنثی اما قطبشپذیر مانند یک سلول، ویروس یا نانوذره در یک میدان الکتریکی ناهمگن اشاره دارد. دی الکتروفورزیس یک ابزار قدرتمند در ریزسیالات و دستگاههای آزمایشگاه-روی-یک-تراشه برای مرتبسازی سلولی است، مانند جداسازی سلولهای سرطانی از سلولهای خونی یا انواع مختلف باکتری. در این مطالعه، یک دستگاه ریزسیال نوآورانه مجهز به الکترودهای حلقوی با ولتاژ پایین ۱ ولت برای کانال اصلی و 0/8 ولت برای کانال فرعی پیشنهاد شده که در فرکانس ۱۰۰ کیلوهرتز عمل میکند. استفاده از ولتاژ پایین، بقا و سلامت سلولهای زیستی را تضمین مینماید که عاملی کلیدی در کاربردهای پزشکی به شمار میرود. نمودارهای پتانسیل الکتریکی، سرعت، فشار و نیروی دی الکتروفورزیس اعمال شده بر سه نوع سلول مورد مطالعه از طریق شبیهسازی ارائه گردید. بازدهی ریزجداکننده پیشنهادی برای سلولهای سرطانی حدود ۹۴٪ محاسبه شده است. در ادامه، با بهرهگیری از روش المان محدود به عنوان یک رویکرد مقایسه ای، تأثیر تغییر ولتاژ الکترودهای کانال اصلی بر جداسازی کارآمد ذرات مورد بررسی و تحلیل قرار گرفت. | ||
| کلیدواژهها | ||
| ریزسیالات؛ مکانیک سیالات؛ دی الکتروفورزیس؛ جداسازی سلولهای سرطانی؛ شبیه سازی | ||
| عنوان مقاله [English] | ||
| Design and Modeling of a Novel Two-stage Dielectrophoresis-Based Microfluidic Microseparator for Efficient Circulating Tumor Cell Separation | ||
| نویسندگان [English] | ||
| Reza Hadjiaghaie Vafaie؛ Elnaz Poorreza | ||
| Department of Electrical Engineering,- faculty Engineering- University of Bonab,- Bonab- Iran | ||
| چکیده [English] | ||
| The reliable separation of circulating tumor cells from blood cells is crucial for early cancer analysis and prediction. The aim of this research is the separation of circulating tumor cells and subtypes of white blood cells (Granulocytes and T-lymphocytes) by employing dielectrophoretic force. Dielectrophoresis refers to the movement of a neutral but polarizable particle, such as a cell, virus, or nanoparticle, in a non-uniform electric field. It is a powerful tool in microfluidics and lab-on-a-chip devices for cell sorting, such as separating cancer cells from blood cells or different types of bacteria. In this study, an innovative microfluidic device equipped with planar electrodes operating at a low voltage of 1 V for the main channel and 0.8 V for the second channel is proposed, which functions at a frequency of 100 kHz. The use of low voltage ensures the survival and health of biological cells, which is a key factor in medical applications. Plots of electrical potential, velocity, pressure, and the dielectrophoretic force applied to the three studied cell types were presented through simulations. The efficiency of the proposed microseparator for cancer cells was calculated to be approximately 94%. Subsequently, using the Finite Element Method as a comparative approach, the impact of changing the voltage of the main channel electrodes on the efficient separation of particles was investigated and analyzed. | ||
| کلیدواژهها [English] | ||
| Microfluidics, Fluid Mechanics, Dielectrophoresis, Cancer Cell Separation, Simulation | ||
| مراجع | ||
|
[1] N.T.A. Othman, F.S.A. Kalam, Blood Cell Tracing in a Microchannel by Using Dielectrophoresis Force, Jurnal Kejuruteraan, 33(1) (2021) 55-61. [2] N.T.A. Othman, H. Obara, A. Sapkota, M. Takei, Measurement of particle migration in micro-channel by multi-capacitance sensing method, Flow Measurement and Instrumentation, 45 (2015) 162-169. [3] J.K. Leal, M.J. Adjobo-Hermans, G.J. Bosman, Red blood cell homeostasis: mechanisms and effects of microvesicle generation in health and disease, Frontiers in physiology, 9(8) (2018) 703. [4] G.J. Kato, F.B. Piel, C.D. Reid, M.H. Gaston, K. Ohene-Frempong, L. Krishnamurti, W.R. Smith, J.A. Panepinto, D.J. Weatherall, F.F. Costa, Sickle cell disease, Nature reviews Disease primers, 4(1) (2018) 1-22. [5] M.A. Mansor, C. Yang, K.L. Chong, M.A. Jamrus, K. Liu, M. Yu, M.R. Ahmad, X. Ren, Label-free and rapid microfluidic design rules for circulating tumor cell enrichment and isolation: a review and simulation analysis, ACS omega, 10(7) (2025) 6306-6322. [6] W. Tang, D. Jiang, Z. Li, L. Zhu, J. Shi, J. Yang, N. Xiang, Recent advances in microfluidic cell sorting techniques based on both physical and biochemical principles, Electrophoresis, 40(6) (2019) 930-954. [7] S.M. Zareei, M. Jamshidian, S. Sepehrirahnama, S. Ziaei-Rad, A Review of Studies on the Motion of Particles Under the Influence of Acoustic Waves in Microfluidic Systems, Amirkabir Journal of Mechanical Engineering, 52(7) (2019) 1905-1924. [8] M. Mehdipoor, H. Badri Ghavifekr, Design and simulation of a biosensor based on a microelectromechanical resonator array, Amirkabir Journal of Mechanical Engineering, 53(6 (Special Issue)) (2021) 3841-3854. [9] B. Techaumnat, N. Panklang, A. Wisitsoraat, Y. Suzuki, Study on the discrete dielectrophoresis for particle–cell separation, Electrophoresis, 41(10-11) (2020) 991-1001. [10] F. Shiri, H. Feng, B.K. Gale, Passive and active microfluidic separation methods, Particle Separation Techniques, (2022) 449-484. [11] S. Chakraborty, Electrokinetics with blood, Electrophoresis, 40(1) (2019) 180-189. [12] A.A. Ayash, H.H. Al-Moameri, A.A. Salman, A.A. Lubguban, R.M. Malaluan, Analysis and Simulation of Blood Cells Separation in a Polymeric Serpentine Microchannel under Dielectrophoresis Effect, Sustainability, 15(4) (2023) 3444. [13] J. Niu, S. Lin, Y. Xu, S. Tong, Z. Wang, S. Cui, Y. Liu, D. Chen, D. Cui, A stepwise multi-stage continuous dielectrophoresis separation microfluidic chip with microfilter structures, Talanta, 279 (2024) 126585. [14] M. Lan, F. Yang, Applications of dielectrophoresis in microfluidic-based exosome separation and detection, Chemical Engineering Journal, 491(10) (2024) 152067. [15] S.M. Tabarhoseini, A.S. Kale, P.M. Koniers, A.C. Boone, J. Bentor, A. Boies, H. Zhao, X. Xuan, Charge-Based Separation of Microparticles Using AC Insulator-Based Dielectrophoresis, Analytical Chemistry, 96(33) (2024) 13672-13678. [16] T. Zhou, J. He, Z. Wu, Q. Bian, X. He, S. Zhou, J. Zhao, T. Wu, L. Shi, H. Yan, Dielectrophoretic–inertial microfluidics for Symbiodinium separation and enrichment, Physics of Fluids, 36(3) (2024) 032018. [17] V. Kordzadeh-Kermani, S.N. Ashrafizadeh, M. Madadelahi, Dielectrophoretic separation/classification/focusing of microparticles using electrified lab-on-a-disc platforms, Analytica Chimica Acta, 1310 (2024) 342719. [18] S.H. Lin, T.C. Su, S.J. Huang, C.P. Jen, Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer‐based surface modification, Electrophoresis, 45(11-12) (2024) 1088-1098. [19] M. Aghaamoo, A. Aghilinejad, X. Chen, J. Xu, On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells, Electrophoresis, 40(10) (2019) 1486-1493. [20] P.R. Gascoyne, S. Shim, Isolation of circulating tumor cells by dielectrophoresis, Cancers, 6(1) (2014) 545-579. [21] S. Shim, K. Stemke-Hale, J. Noshari, F.F. Becker, P.R. Gascoyne, Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems, Biomicrofluidics, 7(1) (2013) 011808. [22] K.A. Hyun, H.I. Jung, Microfluidic devices for the isolation of circulating rare cells: A focus on affinity‐based, dielectrophoresis, and hydrophoresis, Electrophoresis, 34(7) (2013) 1028-1041. [23] E. Poorreza, An Electrokinetic-Based Microfluidic Separator Having Focuser Electrodes for Blood Cells Separation, Transactions on Electrical and Electronic Materials, 26 (4) (2025) 1-11. [24] L. Qin, X. Liu, F. Fei, CFD‐Based Optimization of a Dielectrophoretic Device to Isolate CTCs, Electrophoresis, 46(7-8) (2025) 433-451. [25] C. Yang, Z.-a. Zhou, J. Chen, A.S. Miandoab, M.R. Ahmad, K. Liu, Y. Qin, X. Liu, M. Yu, X. Ren, Circulating Tumor Cells Isolation by a Novel Hybrid Dielectrophoresis-Spiral Microchannel, in: 2025 IEEE 20th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS), IEEE, 2025, pp. 259-264. [26] X. Ou, P. Chen, B.-F. Liu, Liquid Biopsy on Microfluidics: From Existing Endogenous to Emerging Exogenous Biomarkers Analysis, Analytical Chemistry, 97(16) (2025) 8625-8640. [27] V. Varmazyari, H. Habibiyan, H. Ghafoorifard, M. Ebrahimi, S. Ghafouri-Fard, A dielectrophoresis-based microfluidic system having double-sided optimized 3D electrodes for label-free cancer cell separation with preserving cell viability, Scientific reports, 12(1) (2022) 12100. [28] M.R. Uddin, X. Chen, DESIGN OF A HYBRID-INERTIAL DEVICE FOR THE SEPARATION OF CIRCULATING TUMOR CELLS, in: Frontiers in Biomedical Devices, American Society of Mechanical Engineers, 2023, pp. V001T002A002. [29] M.T. Sarowar, M.S. Islam, X. Chen, Separation of CTCs From Blood Cells Using Curved Contraction-Expansion Microchannel Equipped With DEP Force, in: ASME International Mechanical Engineering Congress and Exposition, American Society of Mechanical Engineers, 2023, pp. V012T013A026. [30] T.H. Nguyen, H.T. Nguyen, M.C. Nguyen, L. Do Quang, J. Ducrée, T.C. Duc, T.T. Bui, Circulating Tumor Cell (CTC) Separation from Blood Constituents Using A Dielectrophoresis-Based Microdevice, in: 2024 Tenth International Conference on Communications and Electronics (ICCE), IEEE, 2024, pp. 167-171. [31] R. Agashe, R. Kurzrock, Circulating tumor cells: from the laboratory to the cancer clinic, Cancers, 12(9) (2020) 2361. [32] I. Tivig, L. Vallet, M.G. Moisescu, R. Fernandes, F.M. Andre, L.M. Mir, T. Savopol, Early differentiation of mesenchymal stem cells is reflected in their dielectrophoretic behavior, Scientific Reports, 14(1) (2024) 4330. [33] E. Poorreza, Computer-assisted modeling and simulation of a dielectrophoresis-based microseparator for blood cells separation applications, Chromatographia, 88(3) (2025) 225-242. [34] Y. Zhang, X. Chen, Blood cells separation microfluidic chip based on dielectrophoretic force, Journal of the Brazilian Society of Mechanical Sciences and Engineering, 42 (2020) 1-11. [35] W. Pakhira, R. Kumar, F.C. Panwala, K.M. Ibrahimi, Microfluidic Design for Continuous Separation of Blood Particles and Plasma Using Dielectrophoretic Force Principle, Computer Assisted Methods in Engineering and Science, 30(3) (2023) 323–345. [36] M.S. Bakhshi, M. Rizwan, G.J. Khan, H. Duan, K. Zhai, Design of a novel integrated microfluidic chip for continuous separation of circulating tumor cells from peripheral blood cells, Scientific Reports, 12(1) (2022) 17016. [37] N. Piacentini, G. Mernier, R. Tornay, P. Renaud, Separation of platelets from other blood cells in continuous-flow by dielectrophoresis field-flow-fractionation, Biomicrofluidics, 5(3) (2011) 34122. [38] A.M. Alkhaiyat, M. Badran, Numerical simulation of a lab-on-chip for dielectrophoretic separation of circulating tumor cells, Micromachines, 14(9) (2023) 1769. [39] N.-V. Nguyen, T. Le Manh, T.S. Nguyen, V.T. Le, N. Van Hieu, Applied electric field analysis and numerical investigations of the continuous cell separation in a dielectrophoresis-based microfluidic channel, Journal of Science: Advanced Materials and Devices, 6(1) (2021) 11-18. | ||
|
آمار تعداد مشاهده مقاله: 183 تعداد دریافت فایل اصل مقاله: 251 |
||